Professor Emeritus Ph.D. (1972) University of Colorado Fellow, American Association for Advancement of Science Inventor's Award, University of Georgia, 2007 Executive Editor, Analytical Biochemistry Research Interests - Repetitive DNA landscape of the avian genome: Birds are unusual in the sense that they have one of the smallest genomes among vertebrates with only about 15% repetitive DNA. We have characterized the repetitive DNA fractions generated by Cot-based sequencing and discovered several new transposons as well as adding 5 new family members to the non-LTR retrotransposon, CR1. This work will continue by analyzing additional avian genomes in a wide range of clads to discover when each element entered the genome. Development of the hen oviduct as a bioreactor for the production of exogenous proteins in egg whites: Current emphasis is on developing methods to modify the chicken genome via transgene addition and gene targeting and includes identification of growth factors and cytokines involved in avian germ cell emergence and development. Issued Patents: Production of exogenous proteins in egg whites of transgenic chickens, Robert D. Ivarie, Alex J. Harvey, Julie A. Morris, Guodong Liu, Jeffrey C. Rapp. (US Patent #8,519,214, issued 8/27/13) Transgenic chickens producing exogenous proteins in eggs, Robert D. Ivarie, Alex J. Harvey, Julie A. Morris, Gudong Liu, and Jeffrey C. Rapp. (US Patent #8,507,749, issue 8/13/13) Method for producing exogenous protein in the egg of a chicken, Glycosylated G-CSF obtained from a transgenic chicken, Robert Ivarie, Harvey; Alex J., Morris; Julie A., Liu; Guodong and Rapp; Jeffrey C. (US Patent #7,875,762, issued 1/25/11) Glycosylated human G-CSF, Robert Ivarie, Harvey; Alex J., Morris; Julie A., Liu; Guodong and Rapp; Jeffrey C. (US Patent #7,812,127, issued 10/12/10) Glycosylated interferon alpha. Robert Ivarie, Alex J. Harvey, Julie A. Morris, Guodon Liu, and Jeffrey C. Rapp, (US Patent #7,803,362, ussued 9/28/10)Chicken ovalbumin nucleotide sequence, Leavitt; Markley C., Rapp; Jeff, Ivarie; Robert, and Karnuah; Arthur (US Patent #7,585,963, issued 9/8/09) Avians expressing heterologous proteins, Ivarie; Robert D., Harvey; Alex J., Morris; Julie A., Liu; Guodong , Rapp; Jeffrey C. (US Patent #7,534,929, issued 5/19/09) Glycosylated G-CSF obtained from a transgenic chicken Robert Ivarie, Harvey; Alex J., Morris; Julie A., Liu; Guodong and Rapp; Jeffrey C. (US Patent #7,511,120, issued 3/31/09) Glycosylated interferon alpha. Robert Ivarie, Alex J. Harvey, Julie A. Morris, Guodon Liu, and Jeffrey C. Rapp, (US Patent #7,445,774, issued 11/4/08) Glycosylated interferon alpha obtained from a transgenic chicken, Robert Ivarie, Alex J. Harvey, Julie A. Morris, Guodong Liu, and Jeffrey C. Rapp, (US Patent # 7,338,654, issued 3/4/08) Poultry Derived Glycosylated Interferon Alpha 2b, Robert Ivarie, Jeffrey Rap, & Alex Harvey, (US Patent # 7,129,390, issued 10/31/06) Avian Mago Nashi Gene, Inventors: Robert Ivarie and Tracy Andacht, (U.S. Patent #6,951,736) Vectors in Avian Transgenesis, Inventors: Robert Ivarie, Alex J. Harvey, Julie A. Morris, and Guodong Liu, (U.S. Patent #6,730,822, issued 5/4/04) Method for Windowing Eggs, Inventors: Tracy Andacht and Robert Ivarie, (U.S. Patent #6,397,777, issued 6/5/02) Method for Manipulating Avian Eggs, 1997, Inventors: Gordon Speksnijder and Robert Ivarie, (U.S. Patent #5,897,998, issued 4/27/99) Selected Publications Selected Publications: Wicker, T., J.S. Robertson, S.R. Schulze, F.A. Feltus, V. Magrini, J.A. Morrison, E.K. Mardis, R.K.Wilson, D.G Peterson, A.H. Paterson, and R. Ivarie. 2005. Repetitive landscape of the chicken genome. Genome Res. 15: 126-136. Chicken Genome Consortium (1 of 175 coauthors). 2004. Sequence and comparative analysis of the chicken genome provde unique perspectives on vertebrate evolution. Nature 432: 695-716. Andacht, T. and R. Ivarie. 2004. A rapid and improved method for windowing eggs accessing the stage X chicken embryo. Mol. Reprod. Dev. 69: 31-34. Mott, I. and R. Ivarie. 2004. cDNA array analysis of Japanese quail lines divergently selected for four-week body weight. Poult. Sci. 83: 1524-1529. Ivarie, R. and A.J. Harvey 2003. Validation of the hen as a bioreactor for the production of exogenous proteins in egg white. Poult. Sci. 82: 927-930. Rapp, J., W. Hu, A.J. Harvey and R. Ivarie. 2003. Transgenic hens producing biologically active interferon alpha-2b in egg whites stably through three generations. Transgenic Res. 12: 569-575. Ivarie, R. 2003. Avian transgenesis: progress toward the promise. Trends in Biotechnol. 21: 14-19. Mott, I. and R. Ivarie. 2002. Expression of myostatin is not altered in lines of poultry exhibiting myofiber hyper- and hypoplasia. Poult. Sci., 81: 799-804. Harvey, A.J., G. Speksnijder, L. Baugh and R. Ivarie. 2002. Expression of exogenous protein in the egg white of transgenic chickens. Nature Biotechnol. 19: 396-399. Harvey, A.J., G. Speksnijder, L.R. Baugh, J.A. Morris and R. Ivarie. 2001. Consistent production of transgenic chickens using replication-deficient retroviral vectors and high-through screening procedures. Poult. Sci. 81: 202-212. Speksnjider, G. and R. Ivarie. 2000. A modified method of shell windowing for producing somatic or germline chimeras in fertilized chicken eggs. Poult. Sci. 79: 1430-1433.
